Extrahelical bases
WebApr 26, 2006 · The left (L) and right (R) IntI-binding sites are boxed and the extrahelical bases are shown. The inactive IntI monomers (purple) bound to L are inactivated by conformational changes resulting... WebSpontaneous flipping of a single DNA base to an extrahelical position is a fundamental issue in DNA biophysics. The dynamics of such spontaneous flipping of a lesion or mismatched base is an important biophysical feature of DNA to discriminate damaged base from normal Watson–Crick paired ones.
Extrahelical bases
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WebJan 1, 2007 · The U.S. Department of Energy's Office of Scientific and Technical Information WebFeb 6, 2009 · These studies suggest that DNA base damage can be located by repair proteins by using two mechanisms: a repair protein can probe and detect a weakened base pair that results from mutagenic or cytotoxic base damage; alternatively, a protein can passively capture and stabilize an extrahelical base lesion.
WebJun 3, 2014 · Spontaneous flipping of a single DNA base to an extrahelical position is a fundamental issue in DNA biophysics. The dynamics of such spontaneous flipping of a lesion or mismatched base is an important biophysical feature of DNA to discriminate damaged base from normal Watson–Crick paired ones. WebAug 19, 2007 · The implication was that spontaneous thermally induced opening of T•A and U•A base pairs exposes an extrahelical state that sets in motion the remainder of the base-flipping process.
WebOct 25, 2024 · When the proper base is flipped or not flipped, as encoded by the specific interactions of the finger loop with DNA, the downstream effect of the SRA-DNA interaction is recognized. Our analysis of published SRA–DNA complexes revealed a variety of extrahelical bases, bound DNA sequences, and SRA:DNA stoichiometries (Table 1). In … WebFeb 8, 2011 · Each of these enzymes uses an extrahelical base recognition mechanism, and previous studies with UNG have shown that extrahelical recognition is facilitated by destabilized base pairs possessing kinetically enhanced base pair opening rates.
WebApr 27, 2006 · The extrahelical base G20″ is buried in a deep hydrophobic pocket, located in subunits A/C bound to the other VCR bs duplex, thus forming a set of trans -interactions that hold the synaptic...
WebFeb 20, 2007 · The enzyme uracil DNA glycosylase (UNG) excises unwanted uracil bases in the genome using an extrahelical base recognition mechanism. Efficient removal of uracil is essential for prevention of C-to-T transition mutations arising from cytosine deamination, cytotoxic U*A pairs arising from incorporation of dUTP in DNA, and for … fortress defended by he-manWebAbstract. The efficient enzymatic detection of damaged bases concealed in the DNA double helix is an essential step during DNA repair in all cells. Emergent structural … fortress development solutions greeley coWebFeb 13, 2009 · How some repair proteins, such as AGT (see figure), locate base lesions in DNA is still not fully understood.To remove a few damaged bases efficiently from the context of the entire genome, the DNA base repair proteins rely on remarkably specific detection mechanisms to locate base lesions. fortress development bellevue waWebJan 5, 2024 · extrahelical (not comparable) Beyond, or temporarily removed from the double helix, of a nucleic acid molecule; Usage notes . Used especially to describe … dinner theater near jackson msWebAug 5, 2008 · The astonishingly efficient location and excision of damaged DNA bases by DNA repair glycosylases is an especially intriguing problem in biology. One example is … fortress development incWebApr 27, 2006 · These extrahelical bases, one of which is required for recombination in vivo, originate from folding of the bottom strand of attC … dinner theater near harrisburg paWebFig. 3 Global architecture of the Fpg extrahelical base binding pocket. As indicated, the cFapydG (green)-containing DNA (gray) is stabilized in extrahelical conformation in a large Fpg cavity. The accessibility surfaces of the protein clearly show that this cavity is highly exposed to water molecules. dinner theater near dc