2024 Phis2

Phis2

Author: bnel

August undefined, 2024

Webb酵母单杂. 使用pHIS2载体，将启动子序列构建在HIS3报告基因前面的多克隆位点上，对应的转录因子构建在AD载体上即可。. 使用Y187菌株，它相对于AH109菌株在基因组上少 … Webb19 sep. 2024 · These sequences were cloned into pHIS2 (Clontech, Palo Alto, CA, USA) upstream of the reporter gene HIS3. Co-transformation of pHIS2 with or without the TCP cis-element sequences and GbTCP4-pGADT7 into Y187 cells was performed as described by Yu et al. (2024) . The pGADT7 vector carries the LEU2 nutritional marker for selection …

pHIS2 vector map and sequence

Webb说明：文章内容仅供预览，部分内容可能不全，需要完整文档或者需要复制内容，请下载word后使用。下载word有问题请添加微信号:fanwen365或QQ：370150219 处理（尽可能给您提供完整文档），感谢您的支持与谅解。 Webb10 juni 2016 · Sucrose is not only the carbon source for starch synthesis, but also a signal molecule. Alone or in coordination with ABA, it can regulate the expression of genes involved in starch synthesis. To ... cara upload lagu ke spotify

Y187-pHis2系统酵母单杂互作验证试剂盒

WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … WebbShare your videos with friends, family, and the world Webb26 maj 2024 · pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for … cara upload link ke google drive

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Webb2 nov. 2024 · This Public Health Inspection System (PHIS) Industry User Guide is designed for all industry representatives who produce and sell meat, poultry, and egg products … WebbVector Map pGADT7-Rec Vector (Not sold separately) Sold as a part of Cat. Nos. 630490 & 630491 (102016) Page 2 of 4 . Figure 2. pGADT7-Rec Vector cloning site. cara upload pdf ke bloghttp://coolaber.com/Column.asp?Model=Product_Detail&P_ID=1949722590 cara upload pdf ke google drive

"Webb16 feb. 2024 · The product was cloned into the pHIS2 vector for the bait construction. The coding sequence of PhOBF1 with full length was introduced into the pGADT7-Rec vector harboring the GAL4 activation domain, which was regarded as the pray construct. The recombinant plasmids were co-transformed into Saccharomyces cerevisiae strain Y187. " - Phis2

Phis2

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Webb19 sep. 2024 · C-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in … WebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community …

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WebbPhis2 1 Bait Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … WebbpHIS2 Vector Information Clontech Laboratories, Inc. www.clontech.com Protocol No. PT3705-5 2 Version No. PR32049 Notice to Purchaser This product is intended to be …

Webb1 dec. 2024 · AD/pATM1-pHIS2 and AD-ATM1/pATM1-pHIS2 were cotransformed into the Y187 strain and cultured on SD/-Trp/-Leu for 2–3 days at 30 °C. Then, healthy and … WebbpHIS2.1 DNA target TRP1 Kanr HIS3 Figure 2. One-hybrid library construction and screening. Your target bait sequence is cloned upstream of the His3 reporter in pHIS2.1. …

WebbSnapGene Viewer lets you see your data for free with the same rich visualization, annotation, and sharing capabilities as the fully enabled SnapGene software. SnapGene … WebbPHIS2 Login for pricing STARTER FLUOR 4-22W 220-240V 2P SER See Related Products. Standard Features; Additional Features; Part No. PHIS2. Description. STARTER FLUOR 4 …

WebbY187-pHis2系统酵母单杂原理：当猎物蛋白（Prey）结合到诱饵序列（Bait DNA）上，GAL4 AD就会激活下游报告基因HIS3的表达，能够在含有3-AT的培养基上生长，从而确 …

WebbpHis1, pHis2, pHis3 pGST1, pGST2, pGST3 pMBP1, pMBP2, pMBP3: pET44a-c: Novagen: T7 promoter, N-term his tag followed by NusA fusion protein, another his-tag, S-tag, … cara upload podcast ke spotifyWebbY187-pHis2系统酵母单杂原理：当猎物蛋白（Prey）结合到诱饵序列（Bait DNA）上，GAL4 AD就会激活下游报告基因HIS3的表达，能够在含有3-AT的培养基上生长，从而确定DNA和蛋白有相互。. 本方案中检测诱饵酵母菌株的3-AT最佳使用浓度和互作验证都采用了稀释点板的方法 ... cara upload musik ke spotifyWebbCo-transformation of yeast strain Y187 with pGADT7 and pHIS2 . Point-to-point verification. 1) Culture on the corresponding verification plate. 2) Drip X-α-Gal working solution on the … cara upload produk di e kataloghttp://www.honorgene.com/product_list/carrier_library/123112.html cara upload postingan di instagram pcWebbpHIS-Hsv2 was a gift from James Hurley (Addgene plasmid # 42524 ; http://n2t.net/addgene:42524 ; RRID:Addgene_42524) For your References section: Two … cara upload ppt ke google drivehttp://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf cara upload produk di lazadaWebbUSD $185.00. Matchmaker Insert Check PCR Mix 1 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, … cara upload project android studio ke github