Pma and hl-60
WebHL-60 cells are promyeoloblasts isolated from the peripheral blood by leukopheresis from a 36-year-old,White, female with acute promyelocytic leukemia. This cell line can be used in … WebDec 9, 1997 · 1.. IntroductionThe human leukemia HL-60 cell line is a useful model to investigate the mechanism of cell differentiation. HL-60 cells are induced to differentiate into macrophages by PMA 2, 8.This differentiation is accompanied by an overall decrease in the phosphotyrosine level in the cells [3].It has been shown that the expression of PTP1C, …
Pma and hl-60
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WebHL-60 cell variant, HL-525, which is resistant to PMA-induced macrophage differentiation but not to retinoic acid- or 1,25- dihydroxyvitamin D,-induced differentiation (20). This resist- ant cell variant is deficient in PKC-@ gene expression, the most abundant PKC isozyme in the HL-60 cell line (21). PMA resist- WebMay 15, 2024 · Phorbol myristate acetate (PMA) activates autophagy in HL-60 leukemia cells. • PMA induces autophagy through PKC-mediated activation of ERK and JNK. • PMA-activated ERK/JNK induces TFEB/FOXO1/3-dependent autophagy gene transcription. • Beclin-1/BCL2 dissociation contributes to PMA-induced ERK/JNK-dependent autophagy. •
WebDec 22, 1998 · Macrophage maturation in HL-60 cells is initiated upon activation of protein kinase C (PKC), which acts as both a specific receptor and a functional signal … WebMay 1, 1997 · PMA-stimulated HL-60 cells were prepared by the protocol described previously. Washed cells were resuspended in HBS containing 12.5 mmol/L CaCl 2 at a final concentration of 10 6 cells per mL and warmed to 37°C. The cells then received either 10 μmol/L ionomycin or vehicle (0.1% DMSO).
WebDec 22, 1998 · Human myeloid leukemia cell line HL-60 was originally obtained from R. C. Gallo (National Cancer Institute, Bethesda, MD). The differentiation-resistant cell variant HL-525 was obtained by subculturing HL-60 cells 102 times in the presence of increasing concentrations (up to 3 μM) of PMA at 5- to 8-day intervals (12). WebFeb 19, 2024 · NET-formation of PMA-stimulated primary neutrophils and 3 days DMSO-differentiated HL-60 cells. a NET formation by HL-60 cells differentiated with DMSO for 3 …
WebJordan Priestley Mrs Priestley ICT (@mrspriestleyict) on Instagram: "Come and SEE my exhibitor set up for the PMA Seminar 燐 Lots to showcase & share for teachers..." Jordan Priestley Mrs Priestley ICT on Instagram: "Come and SEE my exhibitor set up for the PMA Seminar 🧮 Lots to showcase & share for teachers to use in their classroom!
WebMay 19, 2000 · Morphologic differentiation of HL-60 cells is associated with appearance of RPTPbeta and induction of Helicobacter pylori VacA sensitivity. Phorbol 12-myristate 13 … ishias beltWebHL-60 cells were exposed to 20 n m PMA for the indicated time before addition of 120 n m VacA, and vacuolation was assessed 8 h later. The longer the exposure of HL-60 cells to 20 n m PMA, the greater the vacuolation induced by VacA at 12 h than at 6 h (Fig. 2 ). More … ishiah whiteWebFeb 11, 2024 · Differentiated human promyelocytic leukemia HL‑60 cells (dHL‑60) have been used to study the mechanisms of NETs formation, as neutrophils have a short lifespan … ishiara color vision chartWebHL-60 cells were seeded in 96-well tissue culture plates (2 3 104 cells/well, 100 ml) in RPMI 1640 medium supplemented with 10% FCS and kanamycin (50 mg/ml). The cells were … safe boats international mark vi patrol boatWebNext to THP-1 and U937 cells, ML-2, HL-60 and Mono Mac 6 cells are used in biomedical research. U937 cells are the most frequently used. The basic difference ... 100 ng/ml PMA (162 nM), followed by washing twice with culture media without PMA and a resting period of 24 h (Chanput et al. 2014 ), resulting in macrophages ... safe body mechanics handoutWebThe protocols I have read seem straightforward on differentiating HL-60 cells, however I am not having luck using 1.3% DMSO and/or 1uM ATRA (all trans retinoic acid). I culture in … safe boating checklistWebMay 15, 2024 · We investigated the mechanisms and the role of autophagy in the differentiation of HL-60 human acute myeloid leukemia cells induced by protein kinase C … ishibashitec.co.jp